Shake flask culture of Laccaria laccata, an ectomycorrhizal basidiomycete.
Applied Microbiology & Biotechnology 45: 319-326
Clem Kuek
Department of Biological Sciences,
Faculty of Business and Technology,
The University of Western Sydney, Macarthur,
P.O. Box 555, Campbelltown, N.S.W.
2560,
Austrralia.
e-mail: c.kuek@uws.edu.au
Abstract
Large-scale exploitation of the potential benefits
of ectomycorrhizal fungi in improving plantation yields means
that fermentation techniques for these fungi will be required.
Starting with a base performance on a rich, complex medium, the
effect of variations in some physicochemical culture parameters
on biomass yield was studied. It was possible to reduce the amount
of phosphate salts (to 1/9th) and other ingredients (to 1/3rd)
in the medium. A shaking speed of either 100 or 200 r.p.m. in
an orbital incubator was satisfactory and biomass yield responded
to an increase in carbon substrate (glucose, from 10 and 20 g
L-1) though Yx/s
declined. An increase in inoculum size shortened culture time
but decreased biomass yield. The upper limit in incubation temperature
was between 25 and 30 oC. Biomass yields of about 12
g L-1 DW (Yx/s =
0.63) when supplied 20 g L-1 glucose, and yield of
about 7 g L-1 (Yx/s
= 0.74) when supplied 10 g L-1 glucose were found.
| Introduction | Materials & Methods | Results | Discussion |
Kuek, C (1996) Shake flask culture of Laccaria laccata, an ectomycorrhizal basidiomycete. Appl. Microbiol. Biotechnol. 45: 319-326
Updated Sunday 23 June 1996